30 min
Anaerobic Setup
<0.1%
Residual O₂
15-21%
CO₂ Generated
24 mo
Shelf Life

VELOY 2.5L Anaerobic Gas-Generating Sachet

The VELOY 2.5L anaerobic gas-generating sachet absorbs oxygen and generates carbon dioxide inside a sealed 2.5L anaerobic jar, creating a strict anaerobic atmosphere within 30 minutes. Its gas-phase and microbiological performance has been benchmarked side-by-side against the MGC AnaeroPack-Anaero 2.5L (Mitsubishi Gas Chemical) reference product in an internal laboratory study — with comparable anaerobe recovery and growth performance at half the import price.

VELOY 2.5L Anaerobic Gas-Generating Sachet

Model: VY-AG250 | 10 sachets/pack · For 2.5L anaerobic jars (≤10 plates)

Test Setup

ItemContent
Report No.TEST-AG-S-20260820 · Issued 20-Aug-2026
Test ItemVELOY 2.5L Anaerobic Gas-Generating Sachet (sealed, 2-8°C, within 24-month shelf-life)
Reference ProductMGC AnaeroPack-Anaero 2.5L (Mitsubishi Gas Chemical)
Test StrainsBacteroides fragilis ATCC 25285 · Clostridium perfringens ATCC 13124 · Fusobacterium nucleatum ATCC 25586
ConditionsIdentical 2.5L polycarbonate anaerobic jars · 37°C / 48 h · 5% sheep blood agar & BBE agar · n = 3 per strain
Acceptance CriteriaResidual O₂ <0.5% · good anaerobe recovery · no growth inhibition

Gas-Phase Performance

Time after jar sealedVELOY GroupMGC Reference
0.5 hO₂ 0.39% · CO₂ 15.00%O₂ 0.36% · CO₂ 7.30%
1 hO₂ 0.10% · CO₂ 19.00%O₂ 0.21% · CO₂ 8.40%
2 hO₂ 0.03% · CO₂ 22.55%O₂ 0.14% · CO₂ 8.80%

Finding: VELOY achieves a strict anaerobic condition (O₂ <0.5%) within 30 minutes and reaches very low residual oxygen. Oxygen indicators were fully colourless in both jars.

Anaerobe Recovery (37°C / 48 h)

StrainVELOY (avg. n=3)MGC Reference (avg. n=3)
Bacteroides fragilis ATCC 25285Recovery 2.7×10⁶ CFU/plate · growth score 3.3 · typical morphologyRecovery 2.8×10⁶ CFU/plate · growth score 3.4 · typical morphology
Clostridium perfringens ATCC 13124Recovery 3.1×10⁶ CFU/plate · growth score 3.7 · typical double-zone hemolysisRecovery 3.0×10⁶ CFU/plate · growth score 3.6 · typical double-zone hemolysis
Fusobacterium nucleatum ATCC 25586Recovery 1.9×10⁶ CFU/plate · growth score 3.0 · typical small flat coloniesRecovery 2.0×10⁶ CFU/plate · growth score 3.1 · typical small flat colonies

Finding: For the three tested ATCC strict-anaerobe strains, comparable CFU recovery, colony morphology and growth level. No inhibition observed under this test condition.

Overall Conclusion

Note on Elevated CO₂

Elevated CO₂ (max 22.55%) does not inhibit growth of the tested Bacteroides fragilis, Clostridium perfringens and Fusobacterium nucleatum. However, fastidious species commonly isolated from genital and oral specimens — such as Gardnerella vaginalis, Atopobium vaginae and some Prevotella spp. — may exhibit reduced colony size or lower recovery under high-CO₂ atmosphere. End-user validation using local target clinical isolates is recommended prior to routine use. Disclaimer: this is an in-house laboratory test result; actual performance depends on jar sealing, operation, temperature and sample matrix.

Document Downloads

Ready to experience high-quality domestic anaerobic gas generators?

Apply for a free trial and verify the results yourself. Import-grade quality at domestic prices, saving your lab over 50% on consumable costs annually.